Primarily I have synthesized silver nanoparticles and checked its anti-colon cancer activity. I just wonder, "how the NPs interact with the cells" and what is its mode of entry into the cells. What are the ligands or chemical group that specifically interact? Please suggest.
Here you pose a big question. NP - cell interaction is a matter of research, in my experience NP enter the cells in different ways depending on cell type and NP properties. I reckon you'll have to check in your model directly by yourself. I will suggest, if possible transmission electron microscopy, it will not tell you which ligand(s) are involved but at list you'll be able to determine the mechanism (endocytosis, pinocytosis etc...). In alternative you can try different inhibitors of these processes... At least you'll reduce the possible proteins to screen.
Dear Maurizio,
Yes, I do agree that its a big question, which answer will reveal a lot of facts regarding the NPs. I understood your suggestion regarding the NPs uptake or entrance into the cell and I am working on that.
But I much interested about the interaction the NPs make with the cells. like My queries are like as follows..
1. How naked NPs interact with the cells (cancer cells specificcally)?
2.How the polymer coated NPs interact?
3. What is the case with the drug loaded metallic NPs?
Please help.
Regards
Shakti
Dear Pavel,
Thanks for the suggestion. It would be better if you put more light on the mechanism. How the thiol group interact and with what?
Regards
Shakti
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