Shakti Ranjan Satapathy

11 Questions 45 Answers 0 Followers

Questions related from Shakti Ranjan Satapathy

Urgently looking for Colon cancer cell lines: MC-38 & SL4. Any help???

I am looking for MC-38 (GFP/RFP) also known as colon-38 and SL4 cell lines for my study. If anyone have it in their lab, I would be happy to contact. Note: We got the MC-38. Please ignore the...

11 November 2016 9,517 1 View

What are the cost effective ways to identify and isolate true cancer stem cells?

There are many methods for identifying and isolating breast CSCs but they are quite expensive. Does anyone have any alternate idea for isolating true CSCs? I want to isolate it from patient tissue...

08 August 2016 2,931 16 View

How to understand the behaviour of SF9 cells?

I am new to the insect cell line culture. I am having SF9 cells and I want to transfer the culture from one spinner flask to another. I am aware that,this transfer depends on the cell density. I...

12 December 2015 7,026 1 View

How the nanoparticles recognize the tumor cells and specifically kill them??

How the nanoparticles, e.g. metallic nanoparticles or polymer capper particles specifically kill the tumor cells, leaving the healthy and non-cancerous cells unaffected? What is the mechanism by...

09 September 2015 7,442 5 View

How we can surface functionalize the nanoparticles as per our requirement??

How can I engineer the nanoparticles with the desired functional groups at the surface??

09 September 2015 10,008 2 View

How do nanoparticles interact with cancer cells?

I wonder how nanoparticles interact with the cancer cell. E.g. In case of metallic nanoparticles which chemical group interact with the cell?

09 September 2014 5,328 10 View

How does one determine the concentration of 'drug-loaded nanoparticles'?

I have prepared the drug loaded PLGA nanoparticle. I want to calculate the exact concentration, so that I can carry out further experiments. Please suggest how.

11 November 2013 8,920 11 View

Determining the concentration of drug formulated nano-particles?

I have prepared a drug-formulated nanoparticle. I want to check the exact concentration of the NPs, so that I can conduct the designed experiments. The NPs have been coated with PLGA & PVA.

08 August 2013 7,562 29 View

Bio-Nano Interaction.

Primarily I have synthesized silver nanoparticles and checked its anti-colon cancer activity. I just wonder, "how the NPs interact with the cells" and what is its mode of entry into the cells....

08 August 2013 7,875 4 View

Analysis of Mitochondrial Membrane Potential (MMP) with microscopy.

I want to analyse how a drug is affecting the MMP. So I need to perform the MMP assay with microscopy. I have tried with Mitotracker red dye but have not found promising results. Please suggest...

06 June 2013 3,336 12 View

What are the effective ways to synchronize cells before any cell based experiments?

It is known that people use serum free medium from 2 h to overnight duration for synchronizing cells prior to experiments. My queries are as follows. 1. How serum free medium synchronize the...

01 January 1970 9,587 17 View