11 Questions 45 Answers 0 Followers
Questions related from Shakti Ranjan Satapathy
I am looking for MC-38 (GFP/RFP) also known as colon-38 and SL4 cell lines for my study. If anyone have it in their lab, I would be happy to contact. Note: We got the MC-38. Please ignore the...
11 November 2016 9,575 1 View
There are many methods for identifying and isolating breast CSCs but they are quite expensive. Does anyone have any alternate idea for isolating true CSCs? I want to isolate it from patient tissue...
08 August 2016 3,047 16 View
I am new to the insect cell line culture. I am having SF9 cells and I want to transfer the culture from one spinner flask to another. I am aware that,this transfer depends on the cell density. I...
12 December 2015 7,043 1 View
How the nanoparticles, e.g. metallic nanoparticles or polymer capper particles specifically kill the tumor cells, leaving the healthy and non-cancerous cells unaffected? What is the mechanism by...
09 September 2015 7,453 5 View
How can I engineer the nanoparticles with the desired functional groups at the surface??
09 September 2015 10,023 2 View
I wonder how nanoparticles interact with the cancer cell. E.g. In case of metallic nanoparticles which chemical group interact with the cell?
09 September 2014 5,340 10 View
I have prepared the drug loaded PLGA nanoparticle. I want to calculate the exact concentration, so that I can carry out further experiments. Please suggest how.
11 November 2013 8,933 11 View
I have prepared a drug-formulated nanoparticle. I want to check the exact concentration of the NPs, so that I can conduct the designed experiments. The NPs have been coated with PLGA & PVA.
08 August 2013 7,691 29 View
Primarily I have synthesized silver nanoparticles and checked its anti-colon cancer activity. I just wonder, "how the NPs interact with the cells" and what is its mode of entry into the cells....
08 August 2013 7,890 4 View
I want to analyse how a drug is affecting the MMP. So I need to perform the MMP assay with microscopy. I have tried with Mitotracker red dye but have not found promising results. Please suggest...
06 June 2013 3,347 12 View
It is known that people use serum free medium from 2 h to overnight duration for synchronizing cells prior to experiments. My queries are as follows. 1. How serum free medium synchronize the...
01 January 1970 9,606 17 View