Urgently looking for Colon cancer cell lines: MC-38 & SL4. Any help???

I am looking for MC-38 (GFP/RFP) also known as colon-38 and SL4 cell lines for my study. If anyone have it in their lab, I would be happy to contact. Note: We got the MC-38. Please ignore the...

10 November 2016 9,417 1 View

What are the cost effective ways to identify and isolate true cancer stem cells?

There are many methods for identifying and isolating breast CSCs but they are quite expensive. Does anyone have any alternate idea for isolating true CSCs? I want to isolate it from patient tissue...

07 August 2016 2,758 16 View

How to understand the behaviour of SF9 cells?

I am new to the insect cell line culture. I am having SF9 cells and I want to transfer the culture from one spinner flask to another. I am aware that,this transfer depends on the cell density. I...

11 December 2015 6,949 1 View

How the nanoparticles recognize the tumor cells and specifically kill them??

How the nanoparticles, e.g. metallic nanoparticles or polymer capper particles specifically kill the tumor cells, leaving the healthy and non-cancerous cells unaffected? What is the mechanism by...

08 September 2015 7,389 5 View

How do nanoparticles interact with cancer cells?

08 September 2014 5,259 10 View

How does one determine the concentration of 'drug-loaded nanoparticles'?

I have prepared the drug loaded PLGA nanoparticle. I want to calculate the exact concentration, so that I can carry out further experiments. Please suggest how.

10 November 2013 8,857 11 View

Bio-Nano Interaction.

Primarily I have synthesized silver nanoparticles and checked its anti-colon cancer activity. I just wonder, "how the NPs interact with the cells" and what is its mode of entry into the cells....

07 August 2013 7,802 4 View

Determining the concentration of drug formulated nano-particles?

I have prepared a drug-formulated nanoparticle. I want to check the exact concentration of the NPs, so that I can conduct the designed experiments. The NPs have been coated with PLGA & PVA.

07 August 2013 7,386 29 View

Analysis of Mitochondrial Membrane Potential (MMP) with microscopy.

I want to analyse how a drug is affecting the MMP. So I need to perform the MMP assay with microscopy. I have tried with Mitotracker red dye but have not found promising results. Please suggest...

05 June 2013 3,233 12 View

What are the effective ways to synchronize cells before any cell based experiments?

It is known that people use serum free medium from 2 h to overnight duration for synchronizing cells prior to experiments. My queries are as follows. 1. How serum free medium synchronize the...

01 January 1970 9,520 17 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View