How we can surface functionalize the nanoparticles as per our requirement??

More Shakti Ranjan Satapathy's questions See All

Urgently looking for Colon cancer cell lines: MC-38 & SL4. Any help???

I am looking for MC-38 (GFP/RFP) also known as colon-38 and SL4 cell lines for my study. If anyone have it in their lab, I would be happy to contact. Note: We got the MC-38. Please ignore the...

10 November 2016 9,648 1 View

What are the cost effective ways to identify and isolate true cancer stem cells?

There are many methods for identifying and isolating breast CSCs but they are quite expensive. Does anyone have any alternate idea for isolating true CSCs? I want to isolate it from patient tissue...

07 August 2016 3,259 16 View

How to understand the behaviour of SF9 cells?

I am new to the insect cell line culture. I am having SF9 cells and I want to transfer the culture from one spinner flask to another. I am aware that,this transfer depends on the cell density. I...

11 December 2015 7,092 1 View

How the nanoparticles recognize the tumor cells and specifically kill them??

How the nanoparticles, e.g. metallic nanoparticles or polymer capper particles specifically kill the tumor cells, leaving the healthy and non-cancerous cells unaffected? What is the mechanism by...

08 September 2015 7,491 5 View

How do nanoparticles interact with cancer cells?

I wonder how nanoparticles interact with the cancer cell. E.g. In case of metallic nanoparticles which chemical group interact with the cell?

08 September 2014 5,377 10 View

How does one determine the concentration of 'drug-loaded nanoparticles'?

I have prepared the drug loaded PLGA nanoparticle. I want to calculate the exact concentration, so that I can carry out further experiments. Please suggest how.

10 November 2013 8,962 11 View

Determining the concentration of drug formulated nano-particles?

I have prepared a drug-formulated nanoparticle. I want to check the exact concentration of the NPs, so that I can conduct the designed experiments. The NPs have been coated with PLGA & PVA.

07 August 2013 7,742 29 View

Bio-Nano Interaction.

Primarily I have synthesized silver nanoparticles and checked its anti-colon cancer activity. I just wonder, "how the NPs interact with the cells" and what is its mode of entry into the cells....

07 August 2013 7,930 4 View

Analysis of Mitochondrial Membrane Potential (MMP) with microscopy.

I want to analyse how a drug is affecting the MMP. So I need to perform the MMP assay with microscopy. I have tried with Mitotracker red dye but have not found promising results. Please suggest...

05 June 2013 3,382 12 View

What are the effective ways to synchronize cells before any cell based experiments?

It is known that people use serum free medium from 2 h to overnight duration for synchronizing cells prior to experiments. My queries are as follows. 1. How serum free medium synchronize the...

01 January 1970 9,644 17 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

Can I proceed with Response Surface Methodology (RSM) with only one factor for further optimization?

I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...

29 July 2024 5,015 1 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View

I did hirshfeld surface analysis for molecule but did not find red spot and interaction surface in energy frame work, is it ok for research article ?

In hirshfeld surface calculation i did not find any red spot in hirshfeld surface, is it ok for research article to write?

28 July 2024 2,988 0 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

Why specific capacitance is not proportional with the specific surface area?

Dear researchers, Why specific capacitance is not proportional to the specific surface area? I have material A with a surface area higher than material B, but material B is showing little bit...

26 July 2024 9,156 2 View