How to quantify the benzene by HPLC method?

I want to know how to quantify the benzene by using HPLC method (any isocratic method)? I performed with methanol and water (80: 20) as mobile phase, but i am not getting any incremental height...

10 February 2020 7,888 4 View

What is the change in H2 entropy from gas to liquid phase ?

03 December 2017 8,434 2 View

Proper solvent to dissolve pure cellulose other than ionic liquids so as to used for electrospinning purpose ?

I want to know except ionic liquids and other solvents combination, which solvent dissolve pure cellulose. Pure cellulose means only cellulose and not its derivatives.

05 October 2017 4,626 2 View

Hii all,I want to do a project on probiotics.i want to use S.epidermis as a probiotic treatment for skin pigmentation .can i know the procedures of it

I want to know if it can be a succesful project and if it can be successful then can i know the procedures how to start it.

09 June 2017 4,129 3 View

What are the cost effective ways to identify and isolate true cancer stem cells?

There are many methods for identifying and isolating breast CSCs but they are quite expensive. Does anyone have any alternate idea for isolating true CSCs? I want to isolate it from patient tissue...

09 August 2016 698 15 View

Which banana liquid crystal phases are required for switching?

Can anyone give the detail idea about banana lc phases takes leading role foe switching?

08 January 2015 4,834 3 View

Anti microbial activity of nano particle from plant extract.

Sir i want some releated article to read. Plz help me.

24 September 2010 8,634 1 View

What are the effective ways to synchronize cells before any cell based experiments?

It is known that people use serum free medium from 2 h to overnight duration for synchronizing cells prior to experiments. My queries are as follows. 1. How serum free medium synchronize the...

01 January 1970 3,220 15 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View