We have to take ethanol or methanol for negative control whereas ascorbic acid/ trolox/quercetin are used for positive control in the formula: DPPH scavenging activity (%)= A0-A1/A0, A0= absorbance of negative control and A1 absorbance of sample. Then what will be the blank?
You can follow our honey paper for determine the DPPH.
https://doi.org/10.1007/s13197-019-03714-9
Check https://www.researchgate.net/publication/357206129_Effect_of_Extraction_Methods_on_Polyphenols_Flavonoids_Mineral_Elements_and_Biological_Activities_of_Essential_Oil_and_Extracts_of_Mentha_pulegium_L?_sg%5B1%5D=
https://www.dojindo.co.jp/manual/d678e.pdf
Also check https://www.mdpi.com/2076-3921/8/5/142/pdf
https://onlinelibrary.wiley.com/doi/pdf/10.1002/fft2.10
Kindly check on this paper
Article A new colorimetric DPPH• scavenging activity method with no ...
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