Shakti Ranjan Satapathy

6 Questions 18 Answers 0 Followers

Questions related from Shakti Ranjan Satapathy

Urgently looking for Colon cancer cell lines: MC-38 & SL4. Any help???

I am looking for MC-38 (GFP/RFP) also known as colon-38 and SL4 cell lines for my study. If anyone have it in their lab, I would be happy to contact. Note: We got the MC-38. Please ignore the...

15 November 2016 9,806 1 View

What are the cost effective ways to identify and isolate true cancer stem cells?

There are many methods for identifying and isolating breast CSCs but they are quite expensive. Does anyone have any alternate idea for isolating true CSCs? I want to isolate it from patient tissue...

10 August 2016 884 15 View

How to tag a nano particle with a fluorescent dye?

I want to tag the polymer capped nano particles with a fluorescent dye. Most of the method that I have referred do not work. They are simply not reproducible even though they were published in...

10 August 2016 3,405 3 View

How does one determine the concentration of 'drug-loaded nanoparticles'?

I have prepared the drug loaded PLGA nanoparticle. I want to calculate the exact concentration, so that I can carry out further experiments. Please suggest how.

12 November 2013 5,633 10 View

How to Visualize the coating of a polymer on NPs?

I have synthesized drug formulated NPs. I have coated the NPs with certain polymer. I want to visualize and confirm the coating with the help of microscopy. I have referred to a paper and applied...

11 September 2013 3,771 2 View

What are the effective ways to synchronize cells before any cell based experiments?

It is known that people use serum free medium from 2 h to overnight duration for synchronizing cells prior to experiments. My queries are as follows. 1. How serum free medium synchronize the...

01 January 1970 3,356 15 View