I want to know how to quantify the benzene by using HPLC method (any isocratic method)? I performed with methanol and water (80: 20) as mobile phase, but i am not getting any incremental height change with increasing the concentration of benzene.
Hello, it may stemming from many different variables. One of them is that you might be tryin to analyze high concentrations of benzene and that is why the signal is not responding to concentration increasements. Try to lowering the concentrations of benzene.
You may be saturating the detector, as described above. However, I didn't see much information about your analyte: is it a sample, or a standard that you are running? Make sure the peak you see is, indeed, benzene (run blanks and standards, if you haven't already done so).
Thank you Erhan Sir, but the standard I have tried that is 0.05, 0.1, 0.15, 0.2, 0.25 ppm of benzene. Should I try the concentration less than 0.05 ppm? I have diluted the benzene standard with HPLC grade methanol to prepare the benzene standard. Blank run also I have performed. I am sure that the peak I am getting due to benzene, but when the concentration changing the area is not increasing linearly.
Which type of detector you are using? If it is UV-vis, please make sure that you selected the right wavelength. And the concentration range of yours seems quite narrow, try to increase it (for example 0.01-50 mg/L).
You can also check this article at the DOI number of 2008-5435/11/31-12-17.
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