Unknown protein - FAQS.TIPS

More P.Anusuya Anu's questions See All

which material is used as base for thin film sample preparation in XRD analysis?

kindly guide me to prepare a Thin-film sample of nanoparticles for XRD analysis.

10 January 2021 3,744 4 View

Can the cell line NG108-15 (neuroblastoma x glioma hybrid) form synapses and show network connectivity?

Does it form networks in 3D cell culture (e.g. when grown in collagen matrix)? What other options for neural cell lines (PNS related) exist that show network connectivity? Can the network...

17 September 2020 4,923 2 View

I need help to interpret my cox proportional model?

Hi There I was performing a cox proportional model for a cancer study in SPSS and I had this output where the Exp(B) was 0.0000064 and the CI 0 to 3.69E+51 ; p= 1 How would I report this Thank...

13 August 2019 5,054 3 View

QuPath: How to measure intensity of staining?

Hi, I'm currently working on my steroid receptor project. I have different receptors stained in tissue samples and currently using QuPath programme to analyze the scanned images. My question is,...

15 June 2019 6,489 3 View

Hello, How can I use the D-R and Tempkin isotherm models on the Origin Pro software? How write the equation and use?

Mostly I use the Langmuir, Freundlich and Sips model for the describe the isotherm database but I don't know how to use D-R and Tempkin isotherm models

20 March 2019 4,106 15 View

Whether I need to adjust the PH of percoll used for gradient centrifugation?

Hi, I am going to do Percoll gradient centrifugation for subcellular fractionation. Before I start I would like to know whether I need to adjust the PH of percoll to 7.4. I use percoll from...

19 November 2018 2,393 3 View

Can we changed the manuscript title when it comes to us back for revision?

I have received revision for a manuscript i submitted to a journal...I had done a survey among elderly population in randomly selected 4 districts...the sample size was 600 and was selected from...

26 May 2018 4,188 3 View

How to generalize a survey done for a specific sample?

I have done a survey among 600 elderly living in old age homes. The sample size was estimated using prevalence of previous study and the sample was selected using cluster random sampling. 4...

25 May 2018 2,494 4 View

Is immunoflorescence staining or western blot is more sensitive to study the protein expression?

Is immunofluorescence staining or western blot is more sensitive to study the protein expression?

25 March 2018 5,866 8 View

Can anyone help me with implementing fuzzy SVM in Python?

Can anyone help me implementing fuzzy SVM in Python or any other language? I want to see if the fuzzified SVM yeilds better results than naive SVM for my dataset.

06 March 2018 5,999 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View