I have designed ShRNA oligos and will be ligating in plasmid. I want know if my shRNA will work if I have hUBC promoter in my plasmid? Or do i need to have U6 promoter.
H1 of U6 promotors are prescribed for shRNA constructs as they are driven by pol III which is present endogenously in the cells. so such shRNAs will be expressed constitutively.