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Questions related from Mohammed Hakim Jafferali
Have some one prepared N,N dimethy casein solution. Could you please share how you done. I am looking for method that doesnt use DMSO. The lypholized powder came as 1g in a plastic bottle, I dont...
17 November 2021 3,213 2 View
I am planning to pcr amplify 800bp from genomic DNA. The end product will be PCR purified and sent for sequencing to detect snps . My question is which polymermase to use. should I use phire hot...
07 April 2021 2,471 5 View
Hi, I have designed ShRNA oligos and will be ligating in plasmid. I want know if my shRNA will work if I have hUBC promoter in my plasmid? Or do i need to have U6 promoter.
01 March 2016 9,284 2 View
I am trying to create c2c12 stable cell line. I performed death curve and found 1mg of g418 was optimum for 95 % of cell death and at 1,5 and 2,0 mg no survivors at all. Then I performed...
22 July 2015 6,544 7 View
I have c2c12 cell lines which on serum deprivation or changing to horse serum, the cells differentiate into myotubes. Now am looking for other immortal muscle cell lines like c2c12. It can be...
06 February 2015 2,208 6 View
