Mohammed Hakim Jafferali

4 Questions 21 Answers 0 Followers

Questions related from Mohammed Hakim Jafferali

Polymerase for Genomic dna PCR amplification & sequencing?

I am planning to pcr amplify 800bp from genomic DNA. The end product will be PCR purified and sent for sequencing to detect snps . My question is which polymermase to use. should I use phire hot...

07 April 2021 2,432 5 View

U6 or hUBC promoter plasmid for ShRNA plasmid?

Hi, I have designed ShRNA oligos and will be ligating in plasmid. I want know if my shRNA will work if I have hUBC promoter in my plasmid? Or do i need to have U6 promoter.

01 March 2016 9,241 2 View

Problem with stable cell selection using G418 ?

I am trying to create c2c12 stable cell line. I performed death curve and found 1mg of g418 was optimum for 95 % of cell death and at 1,5 and 2,0 mg no survivors at all. Then I performed...

22 July 2015 6,494 7 View

Can some one suggest immortal mouse muscle cell lines other than c2c12?

I have c2c12 cell lines which on serum deprivation or changing to horse serum, the cells differentiate into myotubes. Now am looking for other immortal muscle cell lines like c2c12. It can be...

06 February 2015 2,167 6 View