Troubleshooting oligodendrocyte APC O4 staining in spinal cord?

03 January 2020 0 5K Report

Hi,

Recently I am trying to stain oligodendrocytes with APC antibody in the mice spinal cord but I only see APC positive cells clearly in young mice below 90days age. Is that normal? I am wondering if fixation variations between mice to mice might cause this antibody to not work well. I will be happy if you are staining successfully oligodendrocytes please share your material and protocol for doing so.

Cheers,

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