I have files with TRINITY id code and differential expressed values as well as the p values after a de novo transcriptome assemply. What is the proccess after this step to identify which are the genes that I have the differential expression for?
check the trinity output directory for Trinity.fasta file and perform a blast using the closest species transcriptome/genome.
I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.
04 February 2021 6,068 2 View
Hello. Someone designed primers and cycler settings for me and it doesn't work. I do hot start PCR with touchdown, I detect Babesia sp. DNA in Acari. The primers are F: GYYTTGTAATTGGAATGATGG, R:...
01 March 2020 3,949 3 View
Are there currentlly any methods developed for determining the differences in genome between two samples?
08 August 2019 4,594 2 View
My cell cultures (HeLa S3, mouse kidney cells, WB-neo, WB-ras) strarted to form clumps during passaging so it is not possible to count them properly. I TRIED 1. make PBS without Mg and Ca...
14 January 2019 9,398 6 View
I use density gradient centrifugation with Percoll to extract bacteria from the bentonite sample. It is working quite well, but I need to remove the Percoll from the bacterial extract at the end...
25 July 2018 981 3 View
Hello, I'm working on my diploma work on thermal oxidation of TiNb alloy.In my work I have oxidized titanium by two procedures thermal oxidation in laboratory atmosphere and in atmosphere of...
22 May 2018 1,704 5 View
Is it nessessary to be inserted in a BAC clone?
05 June 2016 3,512 1 View
01 March 2016 6,885 5 View
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10 October 2012 8,485 11 View
How to increase effectivity of CTL stimulation with synthetic peptide epitopes.
13 October 2011 2,226 1 View
How to manually calculate p value in ANOVA? what is the equation or formula for it?
03 March 2021 4,928 5 View
Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...
03 March 2021 9,989 2 View
I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...
02 March 2021 1,830 3 View
Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...
02 March 2021 7,161 3 View
I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...
01 March 2021 3,610 1 View
Pls share your thoughts
01 March 2021 7,330 4 View
01 March 2021 1,290 2 View
I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...
28 February 2021 4,966 3 View
Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...
25 February 2021 9,936 2 View
25 February 2021 5,713 1 View