I have files with TRINITY id code and differential expressed values as well as the p values after a de novo transcriptome assemply. What is the proccess after this step to identify which are the genes that I have the differential expression for?
check the trinity output directory for Trinity.fasta file and perform a blast using the closest species transcriptome/genome.
I have ordered the SOD colorimetric kit (invitrogen) and a GSH/GSSG ratio assay (abcam, ab205811) and was hoping to use the same brain tissue for both kits. In the methods provided the by the...
11 February 2024 4,390 2 View
Right now I am wondering what I might be possibly doing wrong with my SPSS syntax. I have got 2 already dichotomized variables and I would need to combine them to make the 3rd one. If var1 and...
20 January 2024 579 10 View
I am using MS quadrupole for on-line detection of diluted gases e. g H2 during TPR H2. How can be MS calibrated for quantitative analysis of hydrogen? When I record signal (torr) for 10 % of H2 in...
15 January 2024 7,149 2 View
Hello, I would like to ask a question. I want to do PCR from a large amount of sample, but it does not fit on my plate. This means that in order to test them all, I have to put them on more...
14 January 2024 9,404 1 View
I have a high number of bacterial cultures I am growing, extracting the DNA from, and sequencing as short reads. Due to the format I'm using (96-well plates), cross-sample contamination is a big...
03 September 2023 4,452 3 View
Hello, I wanted to ask if anyone here works with an Opentrons pipetting station. I would like to experience isolating mRNA from cells, for which we also have a thermoblock and a magnetic block,...
23 February 2023 7,659 1 View
We talk about the round oval shape objects. Grid is 15 um. We supossed some kind of drug crystals, but patient has no special medication. Chemistry: blood 2+ leukocytes 2+ pH 7.0 SG 1.009
13 November 2022 112 1 View
Hello, is it possible to syntyhesis sodium tungastate (Na2WO4) using ammoium tungstate or tungstic acid.
01 March 2022 5,872 4 View
I am looking at a monoculture diatom biofilm growth on five different materials measured at 6 different time points. So my variables are: dependent variable is "cells per mm^2"; independent...
20 November 2021 3,473 6 View
I'm running a fermentation in Hungate tube, but I don't know how to calculate total gas volume. I can mesure gas pressure and I get that through this equation (Delta n) = (Delta P) .V / RT I can...
12 October 2021 557 1 View
Is this website real? https://isar.org.in/event/registration.php?id=2434532
08 August 2024 484 1 View
The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...
07 August 2024 9,934 3 View
Program: g_mmpbsa, version 2024.1 Source file: extrn_apbs.cxx (line 152) Fatal error: Failed to execute command: $APBS pybYcUWA.in --output-file=pybYcUW.out
07 August 2024 6,066 0 View
The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.
07 August 2024 9,508 1 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Dear Researchers Kindly share JCPDS 65-7246 file Thanks in advance
04 August 2024 5,613 1 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...
31 July 2024 6,464 5 View