Thank you very much

Isolation and characterization of exosomes from cell culture supernatants and biological fluids from thery http://www.ncbi.nlm.nih.gov/pubmed/18228490. Théry C, Amigorena S, Raposo G, Clayton A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc CellBiol. 2006 Apr;Chapter 3:Unit 3.22.

This unit describes different approaches for exosome purification from various sources, and discusses methods to evaluate the purity and homogeneity of the purified exosome preparations.

What I get to the end of ultracentrifugate is transparent pellet, (unless you aspire well pbs used as a wash can remain colored droplets) resuspended in 20 ul of PBS.

Thank you !

try resuspending in PBS with 0.2%BSA (pre cleared for vesicles). A small amount of protein helps to prevent exo's sticking to each other.

Thank you

Hi

If the exosome pellet is tough to dissolve in pbs you can either let it sit for a few hours at rt or 37c then pipet up and down; or add more buffer and pipet to dissolve; or gently vortex.

kind regards

ketil

Thank you !