Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

How can nanomaterials affect hospital building?

Has high performance concrete affected the construction of the radiology department in hospitals - for example, in its location? The hospital's Proton Radiotherapy department would not have been...

10 January 2020 559 1 View

Presentation level 70 dB nHL to elicit P300 in CI versus normal hearing children?

In a comparative study between normal hearing and cochlear-implanted children, if we presented tonal odd-ball paradigm via loudspeaker to elicit P300 auditory evoked potential: Is the presentation...

10 January 2020 4,776 1 View

How do I create a distribution plot from Mn, Mw and Mz values?

I know that normally the Mw, Mn and Mz values are sought and thus calculated from the distribution curves of GPC/SEC. I, however, need to go the other way. The Software for the GPC calculates...

28 August 2019 8,634 3 View

I have a legal research (the field of labor law) I want to publish, and I do not know about a good publication magazine?

I would like to know the international and regional journals for publication I want to know the websites of the magazines to communicate with. I have a legal research (the field of labor law) I...

22 January 2019 8,486 5 View

Anyone needs a research partner in the field of corpus linguistics?

I'm willing to collaborate in any research in the field of corpus linguistics, Data driven learning or material design as long as the final work will be published in a peer-reviewed journal. my...

18 December 2018 4,394 2 View

Any suggestion on what to research on or topics? (specifically Environmental Finance)?

Still coming up for a title

02 September 2018 5,479 8 View

What methods are most frequently used to assess resource consumption/depletion?

LCA include many impact categories related to emissions but quite few related to use of resources. There are methods that group resources into biotic and abiotic, renewable and non-renewable,...

03 April 2018 1,736 3 View

How is temperature controlled in Mass Concrete for preventing cracking?

How is temperature controlled in Mass Concrete for preventing cracking

05 March 2018 6,914 12 View

How do I quantify DNA using Nanodrop?

how do i quantify dna using nanodrop?

08 August 2016 4,697 5 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View