I use rapid timm staining for mossy fiber. I follow the protocol but I got these bubbles any ideas?
Hello everyone I am new to cryostat and trying to do rapid timm staining. would you please advice me to get a good tissue these are the steps i did 1. perfused with a sodium sulfide solution...
08 June 2024 9,509 3 View
What is the best setup for reference management in large documents? when I write shorter papers I am a very happy Zotero user. However, now I have a 300 pages book manuscript (MS Word, OSX,...
19 April 2024 3,075 1 View
how to do the search and methodology using inclusion criteria, themes, prisma diagram and casp tool
02 February 2024 7,272 1 View
I am doing a research on different Enrofloxacin-containing veterinary products (injectable and oral solutions)and observing the differences . some products are acidic and some are basic. So my...
10 September 2023 135 1 View
I am doing a research on documentation for the research and development department, i have a document about the formulation of a new product but it looks like missing a lot of important...
21 December 2021 3,488 1 View
the survey sample size for an expert sample that is accepted by a Q2 journal
22 September 2021 6,158 4 View
I have a high density product contains 30% Oxytetracycline dihydrate dissolved in pyrrolidone, it is very difficult to filter with vacuum filtration method ( 0.2 or 0.45 micron nylon filter paper)...
05 September 2021 4,718 4 View
The motion performed is drop jump into side step cutting motion. I am having difficulty finding equations for calculating moments from 3d markers (data obtained from vicon) and thus having trouble...
25 July 2021 3,334 3 View
what solvents are the best to maintain buparvaquone activity in a 5% injectable form? is NMP good without the addition of DMSO?
30 May 2021 8,294 2 View
I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...
03 March 2021 3,087 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there, I'm new to modelling with comsol. I would like to ask if it is possible for comsol to output linearly polarized (LP) modes? I tried modelling a simple single mode fiber with an enlarged...
23 July 2024 4,019 3 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View