Trizol contamination? and method

01 January 1970 0 2K Report

I cultured in-vitro bacteria (1X10^8 cells) and I used Trizol to extract the RNA. After I separated the phases I mixed 0.4 mL of the upper RNA phase with cold isopropanol. Before I measured the RNA values with the Nanodrop and these values were rather low. Ratio between 260 and 230 was +- 0.1 to 0.5. Could the Trizol contaminate my samples? When the aqueous phase was collected I did not touch the lower phase. What should I do next?

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