Is 1 kb of DNA strand good for CRISPR?

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How can you calculate the internal energy of a gas from the equation of state?

Let us say that I have an equation of state for a non-ideal gas (i.e. the Vanderwaals equation). I would like to calculate the internal energy of the gas U(V,T) without any 'microscopic input'. 1)...

21 March 2024 2,130 3 View

Which solutions have been proposed to Schroeder's paradox?

Schroeder's paradox states that a polymer(gel) swells more in contact with a pure liquid than the vapor in equilibrium with this liquid. Thermodynamics states that both the liquid and vapor have...

20 September 2021 5,822 0 View

Electrical Impedance Spectroscopy - how to estimate charge carrier density and mobility?

Presently, I am measuring the frequency dependent conductivity and permittivity of a powder subject to water vapor. From my data I would like to estimate the charge carrier density and the...

22 January 2021 8,390 2 View

Does anyone have some practical tips on how to prepare giant Tridacna shells for non-destructive XRF scanning and LA-ICPMS?

Tridacna shell preparation for non-destructive XRF scanning and LA-ICPMS

25 August 2014 377 3 View

Does anyone have good references on how to measure Boron-10 in fossil corals?

13 November 2013 3,551 2 View

Is there an azole specific efflux family in fungi?

It looks like azole transporters are similar in many fungi, saprophytes, plant and human pathogens. Many of hte azole transporters have homology to CDR1, like BcatrD (and a bit B) from Botrytis...

03 April 2013 1,415 1 View

What is a good website to compare lab products

As a technician I sometime want to compare different lab products. There are a lot of website and I am wondering which websites you use?

01 January 1970 5,272 13 View

Trizol contamination? and method

I cultured in-vitro bacteria (1X10^8 cells) and I used Trizol to extract the RNA. After I separated the phases I mixed 0.4 mL of the upper RNA phase with cold isopropanol. Before I measured the...

01 January 1970 2,475 0 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

Why knockouts for same genes from two different gRNA are showing different result?

Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.

25 July 2024 8,313 1 View

Is possible to edit a gene present in a plasmid by Crispr Cas9?

Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...

22 July 2024 7,073 2 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

What is the most effective method for knocking out a gene in Candida albicans?

I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...

15 July 2024 3,977 1 View

Can ROBINS-I be used to measure risk of bias in single-arm studies?

I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...

14 July 2024 3,570 5 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Are there any information about protocols of CRISPR in leishmania strains?

I am looking for a CRISPR protocol for application in leishmania strains

08 July 2024 9,732 1 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

What causes unexplained rash on the whole body after consuming Stillnox and Serdep in patients on Jalramet, Reaptan, Aspavor, Chela-fer and Myoprin?

I want to understand what are the contraindications and side effects caused in diabetic patients who are on chronic medication (Stillnox and Serdep in patients on Jalramet, Reaptan, Aspavor,...

25 June 2024 984 0 View

Muhammad Ali

Please follow : https://academic.oup.com/nar/article/48/2/974/5651333Article Delivering CRISPR: a review of the challenges and approaches

Article Comparative analysis of single-stranded DNA donors to genera...

Roy Rabinowitz

Large insertions usually result in lower HDR rates. To my knowledge, the upper limit is around 2-2.5kb.