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Hello! I want to use CRISPR to replace a large strand of DNA. My strand is a little bit over 1kb, is this a problem? Moreover, what length should the homology arms be for a region this size?
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I cultured in-vitro bacteria (1X10^8 cells) and I used Trizol to extract the RNA. After I separated the phases I mixed 0.4 mL of the upper RNA phase with cold isopropanol. Before I measured the...
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