The protocol for isolating N-type calcium channel in DRGs, as described by Moutal et al. 2020, describes using the inhibitors " Nifedipine (10 μM, L-type); ω-agatoxin GIVA (200 nM, P/Q-type); SNX-482 (200 nM, R-type); and TTA-P2 (1 µM, T-type)."

Does this protocol exclude the possibility that TRPV1 channels, opposed to the proposed Na+ and CaV2.2, as being involved in DRG neuron hyperexcitability subsequent to VEGF-A (165A) incubation?

Any help would be greatly appreciated!