Transfection scheme for Luciferase Assay for HBV enhancer activity?

08 December 2023 1 2K Report

I am supposed to do luciferase assay for HBV enhancers EnhI and EnhII. I am quite new to luciferase assay and never done on hands before. So, I have to test certain protein effect on HBV enhancers through Luciferase activity. If there is anyone familiar with that protocol. I am looking protocol or cell scheme with details on culture amount of cells (HepG2 in 12 well plate), Transfection details and other necessary details. Thank you.

Yoav Lubelsky

You can start with these papers as reference.

https://www.pnas.org/doi/10.1073/pnas.0607837103?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed

Article The "Trojan horse" model-delivery of anti-HBV small interfer...

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