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Questions related from Kamindu Gayashan
I am supposed to do luciferase assay for HBV enhancers EnhI and EnhII. I am quite new to luciferase assay and never done on hands before. So, I have to test certain protein effect on HBV enhancers...
08 December 2023 1,917 1 View
Huh7 cells are more cancerous than HepG2 cells due to the point mutation at p53 tumor suppression gene. And, In HBV expression, HBV can express in Huh7 even at the lack of HBx. (HBV with HBx...
12 July 2023 3,100 0 View
What is the function of using a myc tag in a construct plasmid with interest gene? And, how is it working?
21 April 2023 8,890 1 View
I experienced considerable amount of cytoplasmic vacuolization in my HepAD38 cells after recent passaging. What indicates this? Is there any method to reverse the aberrant morphology of HepAD38 Cells?
03 March 2023 3,465 0 View
I am working with cell lines. Usually, I have to extract total protein as cell lysate and run SDS gel. I want to know what protein concentration is ideal for western blot analysis to load into SDS...
01 March 2023 4,549 5 View
As in the figure, I got a weird B actin band in western blot well 4. The well should contain the positive sample but in that case, also I did not get any band as expected. In B actin also it shows...
10 February 2023 3,699 0 View
I have to use Miniprep and Midiprep for plasmid extraction. But, I need to know what are exact differences and purposes of Mini and Midipreps other than resulting plasmid yield. And, What out of...
01 February 2023 1,497 4 View
I am currently working with HepG2, HepAD38, Huh7 and HepG2-NTCP-K7 cell lines. I want to know what are the correct timeline for those cell lines.
27 January 2023 5,480 3 View
In HBV research, HBV DNA Southern blot preparation is done by lysis of cells and viral particles. In that case, it used cold lysis buffer contained 10mM HEPES and 0.01X NP-40. What are the...
25 January 2023 9,025 1 View
