I want to transfect siRNA into cultured DRG neurons. I read so many papers, most of them use Lenti virus or electroporation for this purpose. I am limited to use only any transfection reagent like lipofectamine, as I have to do a high through put screen in DRG neurons. Does anyone have some experience in transfecting DRG Neurons? Could you share the experience?
Jagadeesh, unfortunately my personal experince just using lipofectamine wasn't the best. I never got past 20% success rates and that seems way too small for high throughput screens, as you say you plan to conduct.
That said - if you have access to an Amaxa station (now Lonza), you may be able to work this out, even in adherent cells as the new platform comes with plates for transfection/electroporation. We've used this device for transfection of primary cortical and hippocampal neurons and it works much better than liptofectamine.
Good luck!
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