Dear colleagues,
I am starting new project for directed evolution of recombinant proteins in our lab. Since I am not really advanced in this field, I am asking if there is any basic tool to predict the result of specific change in the sequence of my targeted protein. Our goal is to improve stability or activity of our enzyme.
Thank you for your answers
Dear Andej
is the structur on PBD (protein data babnk) e available for your protein or for an homologues from an other organism that will allow to you to build a structural model?
Because in this case you can use POPMUSIC
Article PoPMuSiC 2.1: A web server for the estimation of protein sta...
https://soft.dezyme.com/
i used it in the past to predict the effect of mutations on my protein and it worked quite well.
To see if there are structures available of your protein or homologues you can run a BLAST search on PBD.
on my blog: proteocool ( https://proteocool.blogspot.com/ ) at PAGE 6 you can find the video: Simple bioinformatic Tools for protein sequence analysis where you may find some guidelines to perform this BLAST search.
good luck
Manuele
Dear Minich,
You can use ease mm, dynamut, I mutant and provean servers to check the effect of mutations in your sequence.
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