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Questions related from Shivangi ..
I have optima polar star plate reader and I want to measure the fluorescence at Ex390/Em 510 and Ex488/Em510 but the machine does not has the specific wavelength filters. Excitation wavelengths...
03 October 2022 6,231 0 View
Hi, In running bacterial MIC with antibiotics, do we actually need to measure OD values in spectrophotometer and after getting values at particular emission and excitation wavelength how we get...
21 August 2022 2,839 13 View
Hi, Can I use 50mM sodium phosphate buffer pH 8 for purification of a GST tagged protein expressed in E.coli? Earlier I have done the purification in 50mM Tris buffer pH 8 but the protein is...
28 October 2021 5,414 1 View
Hi, I am doing enzyme kinetics of Gtpase and ATPase reaction. In calculating the the parameters I found by plotting the substrate against velocity the enzyme has more Vo values for ATP than GTP....
08 October 2021 3,828 7 View
Hi, The ITC protein (73kda) with ligand (GTP) showed no saturation pattern in the ITC experiment even after changing both concentrations. The pattern remains the same in the repeated experiments....
30 September 2021 6,605 7 View
Hi, In an enzyme kinetic experiment, my result showed a consistent decrease in Vmax by increasing magnesium but Km fluctuated. Till 40mM Magnesium, Km decreases, but it was increases at 60mM...
30 March 2021 3,629 16 View
GDP is the product of GTPase reaction. In the biochemical setup containing enzyme GTP and GDP, GDP acts as an inhibitor as the enzymatic activity was found to decreased. Km increases and vmax and...
04 October 2020 6,606 16 View
