My question is about the RNA-Seq technique. How many reads are there in the cDNA produced from the mRNA molecule and what is the average length of these reads and why are those reads so short?
For an enterprise is very important in order to organize the Project Management process, make a procedure for the Demand Analysis Process. Can you provide me methodogies for this matter? Thanks...
06 June 2024 5,090 1 View
I am interest in know experiences where the organizations implement the Change Management aproach.
20 April 2024 9,175 8 View
I am a Nigerian researcher working on microbial diversity of an African fermented food product. I will appreciate it if l can get a location and price for shotgun metagenomic sequencing.
20 April 2024 2,536 2 View
Hello everyone, I am currently undertaking a research project that aims to assess the effectiveness of an intervention program. However, I am encountering difficulties in locating suitable...
24 March 2024 3,341 2 View
what can we say is the the Impact of Renewable Energy Integration on Grid Reliability in Nigeria , with examples and possible solution
15 February 2024 3,082 1 View
I need scientific articles or expert criteria looking forward to hear from you. Best Regards
26 January 2024 4,512 4 View
I need scientific articles about Change Management related to IT Projects?
22 January 2024 2,514 6 View
The process management is an important quality principle. I would like to know the different methodologies in the scientific literature and best authors in this field.
19 September 2023 984 1 View
I am doing some research in this topic and I need to Know the best authors in this field
10 September 2023 6,484 6 View
I am doing some research in terms of Integrated Management Systems. And this topic is relevant in the research.
05 September 2023 955 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View