My question is about the RNA-Seq technique. How many reads are there in the cDNA produced from the mRNA molecule and what is the average length of these reads and why are those reads so short?
I google searched my name the other day and found out that I am a coauthor (without my consent) of a paper published in a predatory journal. Please, is there a way I could take my name off the...
18 February 2020 5,515 5 View
How to interpret the Raman spectra for epoxy and unknown by identifying the distinct peaks for unknown. I attached the Raman spectra of both compounds below.
06 December 2018 1,206 2 View
27 June 2017 6,055 5 View
15 May 2017 2,538 10 View
Dear all, Can anybody please help me to identify this plant? The picture of the plant was taken from a forest in Amuana Praso in the Eastern region of Ghana. Thanks for your help.
02 May 2016 3,792 6 View
Does anyone know any vocation questionnaire to measure pedagogy or pedagogy skills to previously used?
03 June 2015 6,704 3 View
I'm using Amoa_2R and Amoa_1F primers. After the PCR my gel showed multiple bands for the soil sample that I'm working with. I was expecting only a 481 bp. I tried different annealing temperatures...
18 May 2015 9,358 3 View
I want to know which one is the best for interpretation of logit regression.
29 December 2014 2,696 5 View
05 October 2014 5,786 1 View
I used the Roche LightCycler 480 and MCF-7 breast cancer cell line cDNA samples to construct an efficiency curve for the ABCG2 gene. This is the 2nd repeat of the experiment and although my...
14 April 2012 6,286 21 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...
28 February 2021 9,138 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View