How best to thaw plasma samples for an immunoassay (cytokines)?

When I defrost my plasma samples from -80, I do so on ice, slowly. Then I centrifuge the samples. Upon centrifugation, the plasma samples will have a white sticky substance layer at the top, but...

30 July 2020 4,877 2 View

Different RNAdatase comparison?

Hi, I would like to compare my dataset generated with Illumina system with other datasets published and deposited on GEO. Is free software that allows me to do that? or just R software is enough?

29 September 2019 3,356 3 View

How can I clean glassware from TiO2 nanoparticles?

In my lab we've been synthesizing 10-20 nm TiO2 nanoparticles from Titanium Isopropoxide, and now the glassware, no matter how I clean it with solvents, is marked with a white patina. I could have...

05 May 2019 5,747 10 View

Influenza viruses plaque assay ?

Hi, I want a correct protocol for plaque assay with influenza viruses . I used MDCK CELLS ( 450 000 cell/well ) with different strains and different plates but i didn't see plaque formation but...

17 February 2019 5,686 4 View

Can protein folding affect antibody detection in a western blot?

I have problems with my western blots in that I get no signal. I have tried all the usual changing of ab concentration, different blocking etc but nothing is working. Is it possible that my...

21 October 2018 5,207 6 View

Have anybody here performed an abductive research strategy as a tool for textual interpretation of interviews?

Do you have any concrete suggestes about the methodological tools to handle abduction? I find that sorting an categorizing the data by coding is not satisfactory, and are looking for an...

01 August 2018 3,983 7 View

Is it possible to use regular Tris-Glycine Running buffer (SDS) on a NuPAGE Tris-Acetate 3-8% precast gel?

15 August 2017 8,144 3 View

Protocol for F4/80 IHC on lung samples?

Hi everyone! I am trying to perform IHC for F4/80 (ab6640 abcam) on pfa fixed LUNG samples, but i'm not able to obtain a good staining. Do you know a good protocol? I usually use a concentration...

20 February 2017 2,652 2 View

What is the best method to concentrate PCR product after pooling several samples?

28 June 2015 5,116 11 View

Does anyone know of a good self-report questionnaire for locus of control?

Need it for a friends thesis which sets out to explore who succeeds in getting back to work after having been unemployed for a period of time.

20 December 2013 6,961 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View