Are there any good specialists on the packages "leaps" and "SpaMM" in the R environment for collaboration?

My colleagues and I have done work on the polymorphism of flower color in one of the higher plant groups. But, unfortunately, it was rejected due to incorrect statistical analysis. We are just...

10 December 2020 885 3 View

Does anyone have a good protocol for Ki67 staining for free floating sections?

My antibody didn't work at all. I have mouse brains, 30 um cryosectioned, and tried different concentrations of tween, as well as both formamide and SSC treatment to no avail.

18 December 2016 6,751 3 View

Please help me identify this Diptera. This family Tephritidae?

Collected September 2, 2016 in Donetsk, Ukraine

10 September 2016 930 17 View

Is this DiI staining valid or specific?

I placed DiI crystals into the hilus of P10 mice horizontal sections (vibratome). I think the first image is from the CA3 region, and I can't tell if these are dendrites/axons or background,...

01 February 2016 2,766 17 View

What is the most accurate and affordable pipette company?

27 October 2015 7,879 3 View

Can anybody recommend a good text on proposal writing and literature synthesis?

I am looking for suggestions from experts-what they suggest and students-what worked for you?

05 June 2014 3,721 3 View

Plant organ preservation in RNAlater for next generation transcriptome sequencing?

Does anyone have an RNA preservation protocol that yielded good next generation transcriptome sequencing results from preservation of plant organs in RNAlater (or another preservative) under field...

11 March 2014 1,359 3 View

How to make calculations with taking H2SO4 into account as a solvent in Gaussian 09?

I'd like to calculate relative energies for some molecules with taking a solvent into account. Reaction takes place in sulfuric acid, which is not included in the list of defined solvents in...

15 October 2013 7,150 7 View

How can one activate resting B cells and how can you check if they were really activated?

Dear colleagues, We are going to stimulate human B lymphocytes isolated from PBMCs from blood of healthy subjects (negative selection with MyltenilBiotech magnetic beads). It should be a control...

24 May 2012 2,800 29 View

KR20 for reliability of the survey instrument. Looking for clarifications?

I am working on my thesis and I have a survey that has true/false and multiple choice questions. From what I understand, KR20 is used for dichotomous variables to estimate reliability coefficient....

01 January 1970 789 3 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View