This damage of leaves of Gymnocladus dioicus. Who could do it? Mites? Gall midges? Or maybe it's viral or bacterial diseases?

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How to optimize my MS2-tagged RNA pulldown in mammalian cells?

I am trying to do the affinity purification of MS2-tagged mRNA by capturing it on GST-MCP (MS2 coat protein) immobilized on glutathione beads. I used two approaches: transfecting the construct...

12 June 2024 504 0 View

Could somebody recommend serum-free medium for hybridoma cells cultivation?

We cultivate OKT8 hybridoma cells in complete medium with FBS but FBS complicates downstream processing and final product purification. We are going to use HybridomaPlus, Protein-Free Hybridoma...

01 February 2024 9,799 0 View

Have experience in carrying out restoration ecosystem work?

Share your experience in restoration, restoration of ecosystems, special interest in high mountain systems. Particular interest in the work, methods of implementation and the results obtained.

29 January 2023 8,084 6 View

What is the reason for the different symptoms of Botrytis cinerea infection?

Botrytis cinerea infection leads to the formation of necrotrophic lesions on plant leaves. In some cases (infection with several B. cinerea strains), these lesions are surrounded by the area of...

22 June 2021 5,782 10 View

Are there any good specialists on the packages "leaps" and "SpaMM" in the R environment for collaboration?

My colleagues and I have done work on the polymorphism of flower color in one of the higher plant groups. But, unfortunately, it was rejected due to incorrect statistical analysis. We are just...

10 December 2020 1,129 3 View

Have you studied the issues of increasing the efficiency of the bodies of external municipal financial control?

To increase the effectiveness of the activity of control and accounting bodies, it is necessary to build a model for assessing the effectiveness of the bodies of external municipal financial...

16 March 2020 3,085 3 View

Why does distilled glycerin take on a pink color?

I am currently studying the problem with the quality of distilled glycerin. When stored, it acquires a pink tint (see photo). We analyzed glycerol on a gas chromatography mass spectrometer. In the...

01 April 2019 4,463 8 View

Does anyone know a good online tool (comparable to KOBAS 2.0) for transcriptome analysis?

Until now, I was relying on KOBAS ( KEGG Orthology Based Annotation System), which was quite user-friendly and provided p-values for distinct pathways. However, it seems to be no longer available....

20 February 2018 7,839 1 View

Where can I find the Call for pedagogical conference papers indexed in Scopus?

I am going to submit and publish my scientific paper. The study area is multimodal pedagogy, reading strategies and comprehension. Can you send web page of the Call for papers?

28 January 2018 5,605 0 View

What kind of the methods of forecasting of the economic indicators of an enterprise's activity are the best in variable environment condition?

Dear Colleagues, What do you think about what kind of the methods of forecasting of the economic indicators of an enterprise's activity are the best in variable environment condition? I'm...

14 December 2017 7,572 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View