Think that the reason is sulfhydration of the protein, how can confirm that?

10 October 2019 1 810 Report

Hello all,

I have noticed a shifting to low molecular weight band of protein by Western blotting after incubation with H2S donor (presence vs absence of H2S), I think that the reason is sulfhydration of the protein, how can confirm that?

Which technique will help? any suggestion?

Regards,

Yasir

Yasir Al-Taie

Thanks, Sebastian, I'm working on pig homogenate how can I do that?

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