Think that the reason is sulfhydration of the protein, how can confirm that?

Hello all, I have noticed a shifting to low molecular weight band of protein by Western blotting after incubation with H2S donor (presence vs absence of H2S), I think that the reason is...

09 October 2019 702 1 View

Can anyone provide me some articles (or procedure) on organic acids extraction methods from filtrate of fungi to be measured by GC-mas

05 June 2017 2,482 2 View

If i have a plasmid in the plasmid finder, how can i know its biological significance?

this is my plasmid

11 December 2016 3,315 1 View

Secreated protein in the superntant ?

Dear colleague does anyone know how to purify the secreted protein from leishmania into the superntant. I used leishmania as expression system for my protein and it should be in the superntant, I...

11 November 2016 4,925 4 View

Secreated protein in the superntant ?

Dear colleague does anyone know how to purify the secreted protein from leishmania into the superntant. I used leishmania as expression system for my protein and it should be in the superntant, I...

10 November 2016 1,321 5 View

Hi, I want to purify my protein by using TEV protein. Can anyone help me?

Hi, I want to purify my protein by using TEV protein. Can anyone help me?

09 October 2016 3,911 5 View

Could anyone suggest an enzyme for polyvinyl Alcohol degradation, please?

04 May 2016 9,847 3 View

Could anyone recommend me a very fast degradable polyester (degradation within few hours)? what are factors that control the degradation rate?

Could anyone recommend me a very fast degradable polyester (degradation within few hours)? what are factors that control the degradation rate?

08 September 2015 1,552 4 View

Which types of downscaling methods including dynamical and statistical techniques do you recommend?

See above

03 September 2014 6,335 7 View

Why partial least square is a distribution free statistical method?

Can we really use it for normalized and non-normalized data?

19 February 2014 5,120 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View