this is my plasmid
Feature selection with python for ant colony optimization algorithm
20 June 2022 5,102 1 View
I have different readings of culture using PNPP at 405 nm. how can I know the concentration of the protein depending on these reading
05 May 2021 9,914 4 View
I am working on the purification of bacteriocin, when screening for bacteriocin production by agar plug diffusion method, about 25% of isolates were bacteriocin producer but about 10% were gave...
19 February 2021 2,857 7 View
Hello all, I have noticed a shifting to low molecular weight band of protein by Western blotting after incubation with H2S donor (presence vs absence of H2S), I think that the reason is...
09 October 2019 848 1 View
Looking at Call Data Records CDR network as a social one, how do i find tie strength in such a network with the help of call count and call duration? Can we make a good measure from these two...
17 September 2019 4,429 0 View
Without using a community detection algorithm, suppose that i have a telecom social network of 877 nodes and 37025 edges. Roughly speaking, what would be the distribution of nodes and links? Put...
04 September 2019 1,870 3 View
actually, I am looking for a procedure for extraction method to identify organic acids in fungal filtrate by using GC-mas, but I have problems with extraction procedure because it necessary steps...
05 June 2017 2,629 2 View
using induce resistance to control and managing Pythum using some nano- chemicals such salsalic acid is one of type to control such fungi without contact it, if their any procedure or other...
31 December 2016 8,871 0 View
Dear colleague does anyone know how to purify the secreted protein from leishmania into the superntant. I used leishmania as expression system for my protein and it should be in the superntant, I...
11 November 2016 5,050 4 View
10 November 2016 1,460 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...
23 July 2024 5,296 0 View