Think that the reason is sulfhydration of the protein, how can confirm that?

Hello all, I have noticed a shifting to low molecular weight band of protein by Western blotting after incubation with H2S donor (presence vs absence of H2S), I think that the reason is...

09 October 2019 702 1 View

Can anyone provide me some articles (or procedure) on organic acids extraction methods from filtrate of fungi to be measured by GC-mas

05 June 2017 2,482 2 View

I am seeking for some information or procedure about control Pythum using induced resistance?

31 December 2016 8,721 0 View

Secreated protein in the superntant ?

Dear colleague does anyone know how to purify the secreted protein from leishmania into the superntant. I used leishmania as expression system for my protein and it should be in the superntant, I...

11 November 2016 4,925 4 View

Secreated protein in the superntant ?

Dear colleague does anyone know how to purify the secreted protein from leishmania into the superntant. I used leishmania as expression system for my protein and it should be in the superntant, I...

10 November 2016 1,321 5 View

Hi, I want to purify my protein by using TEV protein. Can anyone help me?

Hi, I want to purify my protein by using TEV protein. Can anyone help me?

09 October 2016 3,911 5 View

Could anyone suggest an enzyme for polyvinyl Alcohol degradation, please?

04 May 2016 9,847 3 View

Could anyone recommend me a very fast degradable polyester (degradation within few hours)? what are factors that control the degradation rate?

Could anyone recommend me a very fast degradable polyester (degradation within few hours)? what are factors that control the degradation rate?

08 September 2015 1,552 4 View

Which types of downscaling methods including dynamical and statistical techniques do you recommend?

See above

03 September 2014 6,335 7 View

Why partial least square is a distribution free statistical method?

Can we really use it for normalized and non-normalized data?

19 February 2014 5,120 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View