For certain reasons, frozen tissues are only for in situ staining. Is it possible to use frozen tissues for other purposes? Such as isolating the tumor cells from the other cell types present for in vitro culture. I value any feedback and discussion on this.
Hello, Many methods exist. Cancer cells have unique mechanical properties that can be tested all-mechanical or optomechanical. Also, cancer cells can also be identified visually by shape or by optical scattering.
If you have the possibility to measure cell hydration is also a way for differentiation. The cancer cell always has higher hydration rate than normal cells
Goog luck!
Добрый день! У меня были случаи культивирования in vitro биоптатов опухолей головного мозга человека после заморозки в условиях морозильника ( -4 С). После заморозки культура опухоли растет немного медленне свежевыделенной, но результат был положительный. Пробуйте!
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining