An activity in which the body is propelled by moving the legs rapidly. Running is performed at a moderate to rapid pace and should be... | Contact experts in Running to get answers
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Hello! I want to prepare RIPA buffer for total protein extraction. Do you have a command to prepare the RIPA buffer?
01 July 2024 2,940 0 View
Hi, I hope you maybe can explain something from the following: Whole Blood collected in EDTA vials have been sent for DNA extraction with a commercial genomic DNA kit and through an...
12 June 2024 5,021 2 View
Hello every body I use CTAB method for Plant DNA extraction and I need DNA for downstream steps. Unfortunately I have problem in my plant DNA extraction, quality and quantity not as expected, and...
11 June 2024 6,936 2 View
I have been struggling to determine the protein concentration, which is crucial for optimizing the working concentration needed for my research assays. Unfortunately, I have been receiving...
30 May 2024 2,333 4 View
Can anyone share their experience of working with Anti-FLAG® M2 Magnetic Beads used for purification of 3x FLAG tagged protein.
26 May 2024 7,789 1 View
Hello there! I'm looking at a mediation model with 2 predictors, 2 mediators and 3 outcomes. My full sample size is 558, but one of those outcomes only has data for 410 participants. So when I...
19 May 2024 1,706 4 View
I have quantified the lysate by bradford method and loaded 25ug of lysate for SDS PAGE and proceeded for western blot. However, upon detection I see a variation in loading control i.e., GAPDH. I...
16 May 2024 3,734 6 View
Although I tried to perform western blotting with protein extract from RBCs, I could not detect the interest of protein due to red background (maybe, heme or hemoglobin ) all over the lane. I...
16 May 2024 6,465 1 View
Hello. I am new to cell culture work. May I know whether these small black dots are contaminants or just cell debris? It's quite confusing because they are not moving. This is different from the...
03 May 2024 8,791 1 View
Hi, all I am doing the liposome flotation assay. In the end, I precipitated the protein and then ran the sds-page. But every time I could not see my protein in the gel, it was almost gone, maybe...
02 May 2024 6,374 7 View
I am doing IL6 ELISA to measure mouse serum IL6, but my ODs are so low even for mouse on two weeks high fat diet. My standard curve is good enough as a positive control.
29 April 2024 9,195 2 View
Guideline to Questions: An Analysis of Data Anonymization Techniques in British Universities This guide provides a general overview of the types of questions I will encounter during the interview....
20 April 2024 4,842 0 View
I have got measurement results from China. But they have given me the results in tfh file format. Can anyone suggest a software to open the file and analyse the results?
15 April 2024 1,112 3 View
we are planning to use UAE for sea urchin gonads, however, we don't know how many gonads will we need to achieve our targeted amount of extract. what is the usual yield of UAE?
10 April 2024 5,105 0 View
For α-type Ti alloys such as some TA series, water queching from temperature above Tβ can induce the formation of acicular α’ martensite within hcp-α matrix. However, the atomic structures of...
08 April 2024 4,603 1 View
Although I can detect the protein (above 200), leaving it for longer in the transfer (plus 2h), I think I am losing my endogenous protein, as its detection is not getting good. How can I solve it?
08 April 2024 2,630 3 View
In male prostitution, the prostitute usually experiences orgasm. This is in contrast to the situation among female prostitution, most of whom go into prostitution for the sake of the money that...
07 April 2024 9,422 0 View
Hi there, I am trying to design primers to detect shRNA in the transfected cells with my vector. The structure of shRNA within the vector is stem-loop-stem, with a terminator downstream of it and...
05 April 2024 4,038 3 View
I began by processing a 500 mg plant sample, grinding it in 5 ml of phosphate buffer. Following this, I centrifuged the mixture for 15 minutes and collected 1 ml of the resulting supernatant. To...
05 April 2024 4,310 1 View
Salt water does not evaporate faster than fresh water; in fact, fresh water always evaporates faster than salt water. This is because of the difference between the salt and water molecules. Water...
01 April 2024 6,593 1 View
Hello, I am currently attempting to obtain amplifications of SoxC from a species of onychophoran belonging to the Peripatidae family using primers designed from a sequence of its sister family,...
31 March 2024 1,868 4 View
Having problems with dimers and an answer from Paul Rutland for another question made me think that the fact that I store my PCR mix in the fridge might be the problem. I must add that in this mix...
29 March 2024 10,171 3 View
Hi I'm trying to calculate the Van Hove correlation using Pymatgen, but I'm unable to get the Van Hove correlation plot. I am using files obtained from AIMD simulation using VASP. Please let me...
20 March 2024 9,007 0 View
when performing EIS for lithium ion batteries (whether it's LCO/LFP/NMC), you're going to get semi-circles followed by a straight line in low frequency regions. there could be 2-3 semi-circles....
18 March 2024 2,231 1 View