We have a mammalian cell expression protein, want to add the TEV cleavage site at the C-terminal, have given the ORF sequence in the oligoperfect designer, and have given TEV site, after checking the TEV amino acid sequence it is giving SLKIQVF sequence at the C terminal.

If I check the cleavage site of TEV enzyme, it is ENLYFQ/S.

Why difference in the TEV enzyme aminoacid cutting sequence given by the program, am unable to comprehend.

thanks