12 Questions 4 Answers 0 Followers
Questions related from G V Girish
If any scientist ihas developed a Immortal heterohybridoma line secreting monoclonal anti-D antibodies, we are keen to collaborate to take the research to clinical level for conducting the...
20 September 2018 7,234 0 View
Hi, We into developing hCG, FSH, LH biosimilar expressing CHO clones. Want to collaborate, or seek consultancy from the experts in the field. regards,
12 September 2018 3,701 0 View
Our lab is working on developing the stable gene expressing clone in DG44 cells,. After transfection, transient gene expression is working, after growing the cells in the media with out GHT and...
29 August 2018 9,381 2 View
We have a mammalian cell expression protein, want to add the TEV cleavage site at the C-terminal, have given the ORF sequence in the oligoperfect designer, and have given TEV site, after checking...
07 June 2017 7,997 1 View
Am looking for pSV2-DHFR plasmid for my work. Cannot afford to purchase the plasmid, if any lab is having please give me few ng of plasmid on the blotting paper.
25 February 2017 7,352 0 View
Am working with cloning, and plasmid has an Sap1 restriction site at Forward and reverse (purchased the cut plasmid from an company, they call it Electracloning) and the insert gene has forward...
15 July 2016 1,703 4 View
Beta hCG expression is not seen, took of native signal peptide (20aa) of beta-hCG and replaced with human albumin signal peptide (18aa). Still B-hCG expression is not seen in the HEK293 cells. We...
16 April 2016 409 2 View
Am looking for GS double knockout from CHO K1 cell line using the CRIPR system, any one can deliver the cell line will get the 1000 USD prize.
03 September 2015 7,745 0 View
why dialysis bad should be heated with 10 mM sodium bicarbonate10 mM Na2EDTA, pH 8.0 to activate it? what actually do these chemicals do?
14 April 2015 9,099 1 View
I want to install cygwin on my laptop windows 7 64bit after downloading from the cygwin website installation is getting hard, It is showing that choose a download site during installation but...
27 February 2015 5,893 4 View
I am doing TA cloning using PTZ57R/T vector. Positive control is getting transformation, but my poly A tailed amplicon after ligation with TA vector is not getting transformed. Why is this? I have...
12 February 2015 1,630 3 View
I have done TA cloning using PTZ57R/T vector, positive Control with PCR insert(kit) and TA vector is getting transformed but I think, my amplicon with PolyA tailing is not getting ligated. I am...
12 February 2015 195 7 View
