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Questions related from G V Girish
If any scientist ihas developed a Immortal heterohybridoma line secreting monoclonal anti-D antibodies, we are keen to collaborate to take the research to clinical level for conducting the...
20 September 2018 7,194 0 View
Hi, We into developing hCG, FSH, LH biosimilar expressing CHO clones. Want to collaborate, or seek consultancy from the experts in the field. regards,
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Our lab is working on developing the stable gene expressing clone in DG44 cells,. After transfection, transient gene expression is working, after growing the cells in the media with out GHT and...
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Am working with cloning, and plasmid has an Sap1 restriction site at Forward and reverse (purchased the cut plasmid from an company, they call it Electracloning) and the insert gene has forward...
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Beta hCG expression is not seen, took of native signal peptide (20aa) of beta-hCG and replaced with human albumin signal peptide (18aa). Still B-hCG expression is not seen in the HEK293 cells. We...
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why dialysis bad should be heated with 10 mM sodium bicarbonate10 mM Na2EDTA, pH 8.0 to activate it? what actually do these chemicals do?
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I am doing TA cloning using PTZ57R/T vector. Positive control is getting transformation, but my poly A tailed amplicon after ligation with TA vector is not getting transformed. Why is this? I have...
12 February 2015 1,589 3 View
I have done TA cloning using PTZ57R/T vector, positive Control with PCR insert(kit) and TA vector is getting transformed but I think, my amplicon with PolyA tailing is not getting ligated. I am...
12 February 2015 159 7 View
