Mirza Muhammad Fahd Qadir

7 Questions 15 Answers 0 Followers

Questions related from Mirza Muhammad Fahd Qadir

Empty Crispr/Cas9 donor plasmid?

Hello! Does anyone have a empty Crispr/Cas9 donor plasmid, or a donor plasmid that contains homology arms for EMX1, AAVS1 or hROSA26?

08 August 2016 3,128 2 View

A good fluorophore conjugated P2Y1 receptor antibody out there?

Has anyone used a good P2Y1 receptor antibody conjugated to 488 or 647? For FACS and cell sorting!! Thanks!!!

08 August 2016 7,761 0 View

What is a good set of antibodies for mesenchymal/fibroblast cells and endothelial cells. Considering I want to eliminate these populations in FACS?

Hello! I want to sort out mesenchymal and endothelial populations (essentially the junk) from a population of cells in live Acinar Pancreatic tissue. Can anyone give me suggestions on what array...

06 June 2016 8,480 1 View

Why am I getting overexposure on my immunoblotting?

Hi! Im performing a westren blot, but my immunoblotting isnt going well. The proteins transfer perfectly on the PVDF membrane, but when I incubate the membrane with my primary abs, the signal...

07 July 2014 2,341 13 View

Is it possible to make a cell lysis buffer without PMSF?

Can someone please tell me how to make a cell lysis buffer without PMSF I have sodium deoxycholate but no PMSF. I also have NP-40. Please tell me an appropriate recipe one which does not include...

07 July 2014 1,793 7 View

In animal models, particularly BALB/c mice, how can I induce arthritic induction using collagen type-II?

Arthritic induction is required by the protein rich in joints.

06 June 2014 5,024 5 View

Hi! An excellent project. I would love to connect with you and discuss our research as well. I feel we may have similar interests. Can we?

Our lab studies the effect of BMP7 on pancreatic-progenitor like cells, however we have not studied the effect of this on bone mineralization. Maybe we could collaborate in this regard!

01 January 1970 7,054 2 View