Does anyone have experience on the PhiX concentration required on NovaSeq for ddRAD or RADseq data?

Dear all, we have been running multiple ddRAD libraries prepared by following the protocol of Petersen et al 2014 in our lab on the HiSeq2500 (SR 100) in the past couple of years. We pooled...

28 September 2020 2,297 1 View

Read counts from RNA-Seq paired end reads?

Hello, I have the following question on paired end reads from RNA-seq: To get the total number of obtained reads from paired end reads (2 fastq files are generated (R1, R2), is it correct to add...

01 September 2020 7,715 5 View

Does anybody have experience with the detection of a FLAG epitope on a Western blot with the Sigma Anti-FLAG antibody M2 after stripping the blot?

13 August 2020 8,401 3 View

How can I test if my TaqMan Probe is annealing?

Hello! I'm trying to perform a TaqMan qPCR to check for certain fish species in environmental DNA samples. I am using a published primer + probe set. The probe is carrying FAM at it's 5' end and...

14 April 2019 4,907 15 View

Stats for dissertation?

I need some guidance on selecting the appropriate statistical methods for my research. I am investigating lone violent extremists by subtype (far right, far left, Islamic and single-issue) on 4...

11 May 2018 1,809 3 View

If I have cultured keratinocytes expressing only K14 gene, is it possible that under certain conditions, they become activated and express K16?

18 December 2014 1,476 6 View

How I can obtained well formed Embryoid Bodies from Embryonic Stem Cells in Serum free medium?

Hello Everyone I tried to differentiate Embryoid bodies (EBs) from mouse Embryonic Stem cells (E14tg2a) following the protocol I attached. However i failed during the process because despite cells...

01 January 1970 155 6 View

Looking for a psychometric assessment tools to measure maturity in adolescents?

Working on a behavioral therapy protocol and looking for a psychometric assessment tool to measure maturity in adolescents and young adults up to 24 years in age.

01 January 1970 3,684 7 View

How to perform Microsatellite data analysis?

Hello, I have an excel sheet with microsatellite data, I would like to make a NJ tree out of it. I have a basic level in bioinfo. Can someone please guide me through the steps to get the...

01 January 1970 6,828 2 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

Where can I find raw quantitative datasets on ecosystem services?

I'm an Undergraduate student on the search for a research topic which has to involve any ecosystem service(s) and a response to environmental change. I'm looking for raw quantitative data sets (I...

19 February 2021 5,911 2 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to find the capacitive response frequency from the impedance plot?

I have done electrochemical impedance by using cyclic voltammetry for one organic material. from that, I plotted bode diagram. but actually, I am very confused about how to find the capacitive...

18 February 2021 3,023 3 View