I did the TEM analysis of CuO nano particles green synthesis, but the images were very bad and the particles agglomerated. Although the XRD is very well. The TEM technician told me that the nanoparticles dissolved in ethanol and sonicated and put on the carbon grid 3 days before the test. How can I know the main reason behind these bad images?
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Aml Mahmoud
The TEM specialist did not wash the nanoparticles well from the molecules on their surface. Wash them several times with ethanol yourself in a centrifuge. Precipitate the nanoparticles, add ethanol again and do this several times. To prepare them on a grid, make a diluted dispersion.
The poor TEM images of CuO nanoparticles may be due to agglomeration during sample preparation, inadequate dispersion, or high concentration on the carbon grid. To improve image quality, optimize sample preparation by reducing the time between sonication and imaging, adjusting nanoparticle concentration, and ensuring adequate stabilization to prevent agglomeration.
The agglomerated TEM morphography of CuO nanoparticles might be due to agglomeration during sample preparation for moisture of long time air expousre, improper dispersion, or impuriies of foreign particle on the carbon coated copper grid. To get high resolution image quality, optimize sample preparation by washig sample ethanol and acetone 5 time at 30 minutes & 10000 rpm, reducing the time between sonication and imaging. when analysis use ingap apperature in TEM when analysis if possible.
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