My friend want to design a study to find the mutation in TLR2, NRAMP-1, VDR and IFN gamma-R using RFLP technique. If any one have experience in it can you please suggest the primers and restriction enzymes required for each genes.
What is the significant fold increase for delta delta ct analysis for a prokaryotic gene. Is it above 2 fold? If so, have any one have got above two fold increase in any prokaryotic gene? Iam...
11 December 2016 5,542 4 View
Is their a particular fold difference or any statistical method to find whether the q pcr result for a treated sample and untreated sample are significant or not?
06 July 2016 5,648 5 View
For significant change it was said FC should be above 2. Is it the same for prokaryotic and Eukaryotic expression analysis? Can some one brief me on this issue and whether it have any relation...
06 July 2016 8,200 2 View
I need to knockout some genes for functional studies. can any one suggest me the best and time saving technique which i can use. One problem i would face is the isolates are resistant to most of...
05 June 2016 2,646 3 View
Let as say their is a susceptible isolate and a resistant isolate, the resistant isolate have more acquired resistant genes. To express those genes more ribosomes are needed and so more...
05 June 2016 576 4 View
will rna get amplified when using it as a template for PCR? -using dna specific primer -with gc% 55 -using high fedility polymerase.
05 June 2016 4,472 8 View
When i did PCR for a house keeping gene for different isolate of Acinetobacter baumannii i got no band and a less band for two isolates. Then i did a gradient PCR to get intense band on both....
03 April 2016 4,062 2 View
I want to know whether the RNA i have isolated from Klebsiella pneumoniae is in good condition. I would also like to know what the four bands are (there should be only 3 bands and 5s is not...
03 April 2016 1,292 6 View
I would like to understand the reason behind the silent mutation and nonsense mutation in house keeping genes. Since I am working with multidrug resistant bacteria, i would like to know if there...
02 March 2016 6,333 5 View
I have done MLST for some isolates of Acinetobacter baumannii which are obtained from ICU scenario in a single month. In which five genes have same clonal number in all isolates while only two...
02 March 2016 7,117 2 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View