Delta delta Ct analysis for prokaryotes?

What is the significant fold increase for delta delta ct analysis for a prokaryotic gene. Is it above 2 fold? If so, have any one have got above two fold increase in any prokaryotic gene? Iam...

11 December 2016 5,381 4 View

How to know whether the result obtained from qPCR are significant or not?

Is their a particular fold difference or any statistical method to find whether the q pcr result for a treated sample and untreated sample are significant or not?

06 July 2016 5,534 5 View

Is fold difference and fold change cut off the same in real time PCR?

For significant change it was said FC should be above 2. Is it the same for prokaryotic and Eukaryotic expression analysis? Can some one brief me on this issue and whether it have any relation...

06 July 2016 8,050 2 View

What is the best gene knockout method in clinical isolates of Pseudomonas and Acinetobacter?

I need to knockout some genes for functional studies. can any one suggest me the best and time saving technique which i can use. One problem i would face is the isolates are resistant to most of...

05 June 2016 2,515 3 View

Will Total RNA get amplified using dna specific primer?

will rna get amplified when using it as a template for PCR? -using dna specific primer -with gc% 55 -using high fedility polymerase.

05 June 2016 4,357 8 View

Will endogenous control vary between different bacterial isolates of same organism?

05 June 2016 472 4 View

Why annealing temperature changes for a same bacteria but different isolate?

When i did PCR for a house keeping gene for different isolate of Acinetobacter baumannii i got no band and a less band for two isolates. Then i did a gradient PCR to get intense band on both....

03 April 2016 3,960 2 View

Can any one suggest the integrity of the RNA gel pic that i have attached?

I want to know whether the RNA i have isolated from Klebsiella pneumoniae is in good condition. I would also like to know what the four bands are (there should be only 3 bands and 5s is not...

03 April 2016 1,163 6 View

How can I analyse codon bias in case of silent mutation in a house keeping gene in microorganisms?

I would like to understand the reason behind the silent mutation and nonsense mutation in house keeping genes. Since I am working with multidrug resistant bacteria, i would like to know if there...

02 March 2016 6,219 5 View

Regarding MLST (Multi Locus Sequence Typing) data in Acinetobacter baumannii using 7 house keeping genes?

I have done MLST for some isolates of Acinetobacter baumannii which are obtained from ICU scenario in a single month. In which five genes have same clonal number in all isolates while only two...

02 March 2016 6,948 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View