Delta delta Ct analysis for prokaryotes?

What is the significant fold increase for delta delta ct analysis for a prokaryotic gene. Is it above 2 fold? If so, have any one have got above two fold increase in any prokaryotic gene? Iam...

11 December 2016 5,381 4 View

How to know whether the result obtained from qPCR are significant or not?

Is their a particular fold difference or any statistical method to find whether the q pcr result for a treated sample and untreated sample are significant or not?

06 July 2016 5,534 5 View

Is fold difference and fold change cut off the same in real time PCR?

For significant change it was said FC should be above 2. Is it the same for prokaryotic and Eukaryotic expression analysis? Can some one brief me on this issue and whether it have any relation...

06 July 2016 8,050 2 View

Will Total RNA get amplified using dna specific primer?

will rna get amplified when using it as a template for PCR? -using dna specific primer -with gc% 55 -using high fedility polymerase.

05 June 2016 4,357 8 View

Will endogenous control vary between different bacterial isolates of same organism?

05 June 2016 472 4 View

Why annealing temperature changes for a same bacteria but different isolate?

When i did PCR for a house keeping gene for different isolate of Acinetobacter baumannii i got no band and a less band for two isolates. Then i did a gradient PCR to get intense band on both....

03 April 2016 3,960 2 View

Can any one suggest the integrity of the RNA gel pic that i have attached?

I want to know whether the RNA i have isolated from Klebsiella pneumoniae is in good condition. I would also like to know what the four bands are (there should be only 3 bands and 5s is not...

03 April 2016 1,163 6 View

Suggestion regarding mutation analysis in TB?

My friend want to design a study to find the mutation in TLR2, NRAMP-1, VDR and IFN gamma-R using RFLP technique. If any one have experience in it can you please suggest the primers and...

03 April 2016 4,137 0 View

How can I analyse codon bias in case of silent mutation in a house keeping gene in microorganisms?

I would like to understand the reason behind the silent mutation and nonsense mutation in house keeping genes. Since I am working with multidrug resistant bacteria, i would like to know if there...

02 March 2016 6,219 5 View

Regarding MLST (Multi Locus Sequence Typing) data in Acinetobacter baumannii using 7 house keeping genes?

I have done MLST for some isolates of Acinetobacter baumannii which are obtained from ICU scenario in a single month. In which five genes have same clonal number in all isolates while only two...

02 March 2016 6,948 2 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View