Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Has anyone used ANSYS Student Software to model light propagation?

I'm wondering if one is able to model light propagation in an optical setup using ANSYS Student Software or if it is only possible to do so using SPEOS, which to my knowledge is not included in...

20 February 2020 1,184 1 View

How do you determine Band Gap for a solid, opaque material?

I have seen many answers to the question on how to determine the band gap of a semi-conductor, but that always implies the use of UV-Vis Spectroscopy. However, the problem with this method is that...

28 January 2020 8,981 7 View

How to be more productive while writing?

Do you have any specific schedule when is the best time to write a publication, an article for a journal or an academic book? When is the best productive time for it? Please, share your experiences

29 December 2019 1,705 4 View

Is it wrong to apply multivariate analysis to a group of samples that are different in nature from different publication (i.e., DNA, RNA and protein)?

I am reviewing a manuscript and was wondering if this was a major experimental flaw. Among the eight publications, three used protein, three used RNA, and two used DNA. Apparently, if the...

02 May 2018 2,536 3 View

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Hello everyone! I have been consulting this webpage http://beast.community/ and http://www.beast2.org/ , but the examples for the use of BEAUti and BEAST are for old versions. The versions of...

02 April 2018 3,942 3 View

What buffer should I add to the Krebs-Henseleit solution to counteract the rise of the PH while using pure oxygen instead of carbogen?

I am currently working with the Langendorff's Isolated Heart System and I have some problems with the Krebs-henseleit solution due to a change we were force to do after we ran out of carbogen and...

04 October 2017 9,914 2 View

Have you COI sequences of Potamotrygon orbigyi?

24 August 2017 6,634 2 View

How many sequences it needed to put in a crown group to calibrate a tree?

17 August 2017 4,155 2 View

What software do you recommend to calculate flow rates?

Hello. I need to calculate flow rates from precipitation and temperature data. What software do you recommend? Thanks.

04 May 2017 605 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View