Although Sysmex DI-60 is a fully automated analyzer, further understanding and analytical processes to ensure the reliability of its functionality are needed concerning the factors and their impact on its sample quality or preparation.
With this, what is the impact of sample quality or preparation variations on the accuracy and consistency of red blood cell morphology characterization by the DI-60 analyzer?
Andrei Cedric Rocero Adolfo
The impact of sample quality or preparation variations on the accuracy and consistency of red blood cell morphology characterization by the DI-60 analyzer include (Oh Joo Kweon et al., 2022):
- Blood Smear Quality - The quality of the blood smear, thickness, uniformity, and intensity of its stain, can influence the ability to accurately identify and classify red blood cell morphologies. Smears that are too thick or thin, unevenly spread, or inadequately stained may lead to misinterpretation of their morphology.
- Cell Distribution - The distribution of red blood cells on the smear, such as clumping or aggregation of cells, can impact the ability of the analyzer to locate and analyze individual cells. Clumped cells may be incorrectly identified or missed, affecting the accuracy of morphology characterization.
- Cell Integrity - The integrity of RBCs in the sample, including factors like cell shape abnormalities, cell lysis, or the presence of artifacts, can affect the analyzer's ability to accurately classify cell morphologies. Damaged or distorted cells may be challenging for the analyzer to categorize correctly.
- Staining Artifacts - Variations in staining techniques or the presence of staining artifacts on the smear can interfere with the analyzer's ability to differentiate between different red blood cell morphologies. Staining artifacts may lead to false-positive or false-negative results in morphology characterization.
- User Proficiency - The proficiency of the user in preparing blood smears and operating the DI-60 analyzer can also impact the analysis. Hence, proper training and following of standardized protocols are a must to ensure reliable results.
1 votes
1 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Bioecology
- Systems Ecology
- Landscape Ecology
- Connectivity
More Angelica Kaye Castro Aycocho's questions See All
Similar questions and discussions