What should be the best method to prepare tissue homogenate from murine urinary bladder frozen in liquid nitrogen?
I have been using Dynabead protein-G for immunoprecipitation of my target protein in a mix of antigen from two different protein samples. My target protein X is supposed to bind to the protein Y...
10 November 2016 235 5 View
These plasmids are accessible through AIDS Reagent Program. These are: 1. pNL4-3 and 2. HIV-1 HXB3/BaL Infectious Molecular Clone (pWT/BaL). I assume that these plasmid encoded viruses will be...
08 September 2016 6,889 3 View
I am trying to optimize Taqman based qRT-PCR and p24 based ELISA to determine the viral titer for HIV-1 in macrophages exposed to HIV-1 BaL. Is there any established protocol for TCID determination?
06 July 2016 3,170 2 View
Can anyone suggest of a suitable cell line, the conditioned media of which could be used as a better replacement of recombinant human M-CSF?
06 July 2016 264 6 View
Any suggestion that the amino acids will lead to any detrimental change on differentiation of monocytes in to macrophages in conjunction with recombinant M-CSF?
06 July 2016 3,002 3 View
In a mammalian cell line, autophagy is induced with respect to control cells, still the level of beclin-1 is very similar in both conditions, where a particular inducer of autophagy was used to...
06 July 2014 6,801 2 View
A particular family of miRNA is unregulated in infected monocyte derived macrophages as validated by microarray as well as Taqman assay. However the gene targets are unregulated in the same...
04 May 2014 2,586 8 View
Science is becoming really compartmentalized and we are all in the race to impose our own specialization to the entire research community in respective field. Isn't it time to pause and think...
02 March 2014 1,298 2 View
The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...
07 August 2024 9,934 3 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...
06 August 2024 662 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View