Suggest a suitable method of tissue homogenate preparation from murine urinary bladder for ELISA?

What should be the best method to prepare tissue homogenate from murine urinary bladder frozen in liquid nitrogen?

05 June 2018 2,717 0 View

Immunoprecipitated protein band shifts downwards in Western blot using Dynabead protein G ?

I have been using Dynabead protein-G for immunoprecipitation of my target protein in a mix of antigen from two different protein samples. My target protein X is supposed to bind to the protein Y...

10 November 2016 144 5 View

Looking for R5 HIV pro viral plasmids?

08 September 2016 6,816 3 View

What the the best method of determine the replication of R5 HIV-1 viruses in human macrophages?

I am trying to optimize Taqman based qRT-PCR and p24 based ELISA to determine the viral titer for HIV-1 in macrophages exposed to HIV-1 BaL. Is there any established protocol for TCID determination?

06 July 2016 3,086 2 View

Can we use essential and non-essential amino acids in RPMI media used for differentiation of human monocyte-derived macrophages?

Any suggestion that the amino acids will lead to any detrimental change on differentiation of monocytes in to macrophages in conjunction with recombinant M-CSF?

06 July 2016 2,913 3 View

Can anyone explain why the Beclin-1 level is similar in control and autophagy inducer treated samples?

In a mammalian cell line, autophagy is induced with respect to control cells, still the level of beclin-1 is very similar in both conditions, where a particular inducer of autophagy was used to...

06 July 2014 6,713 2 View

What happens when microRNA and its corresponding target also get unregulated in the same system?

A particular family of miRNA is unregulated in infected monocyte derived macrophages as validated by microarray as well as Taqman assay. However the gene targets are unregulated in the same...

04 May 2014 2,485 8 View

Is it really necessary to make every problem a bombshell of information and then force it upon the research community and young investigators?

Science is becoming really compartmentalized and we are all in the race to impose our own specialization to the entire research community in respective field. Isn't it time to pause and think...

02 March 2014 1,218 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View