Alok Kumar Singh

9 Questions 17 Answers 0 Followers

Questions related from Alok Kumar Singh

Suggest a suitable method of tissue homogenate preparation from murine urinary bladder for ELISA?

What should be the best method to prepare tissue homogenate from murine urinary bladder frozen in liquid nitrogen?

06 June 2018 2,767 0 View

Immunoprecipitated protein band shifts downwards in Western blot using Dynabead protein G ?

I have been using Dynabead protein-G for immunoprecipitation of my target protein in a mix of antigen from two different protein samples. My target protein X is supposed to bind to the protein Y...

11 November 2016 218 5 View

Looking for R5 HIV pro viral plasmids?

These plasmids are accessible through AIDS Reagent Program. These are: 1. pNL4-3 and 2. HIV-1 HXB3/BaL Infectious Molecular Clone (pWT/BaL). I assume that these plasmid encoded viruses will be...

09 September 2016 6,866 3 View

Can we use essential and non-essential amino acids in RPMI media used for differentiation of human monocyte-derived macrophages?

Any suggestion that the amino acids will lead to any detrimental change on differentiation of monocytes in to macrophages in conjunction with recombinant M-CSF?

07 July 2016 2,976 3 View

What the the best method of determine the replication of R5 HIV-1 viruses in human macrophages?

I am trying to optimize Taqman based qRT-PCR and p24 based ELISA to determine the viral titer for HIV-1 in macrophages exposed to HIV-1 BaL. Is there any established protocol for TCID determination?

07 July 2016 3,144 2 View

Any cell line that can be a good source of human M-CSF?

Can anyone suggest of a suitable cell line, the conditioned media of which could be used as a better replacement of recombinant human M-CSF?

07 July 2016 241 6 View

Can anyone explain why the Beclin-1 level is similar in control and autophagy inducer treated samples?

In a mammalian cell line, autophagy is induced with respect to control cells, still the level of beclin-1 is very similar in both conditions, where a particular inducer of autophagy was used to...

07 July 2014 6,777 2 View

What happens when microRNA and its corresponding target also get unregulated in the same system?

A particular family of miRNA is unregulated in infected monocyte derived macrophages as validated by microarray as well as Taqman assay. However the gene targets are unregulated in the same...

05 May 2014 2,550 8 View

Is it really necessary to make every problem a bombshell of information and then force it upon the research community and young investigators?

Science is becoming really compartmentalized and we are all in the race to impose our own specialization to the entire research community in respective field. Isn't it time to pause and think...

03 March 2014 1,272 2 View