Separation of protein via Akta system gave broad peak during elution. I wonder is it normal to obtain broad peak ?

Greetings.. I am using Akta Pure system for my protein purification. I have optimized buffer system for protein elution. However, this time I got broad peak while eluting sample. For...

24 December 2020 393 7 View

While running 15% SDS gel, I have noticed some blurred blue coloration above sample protein. What it could be?

While running 15% SDS gel, I have noticed some blurred blue coloration above sample protein. I have attached figure for the convenience. Suggestion and answers in this regard will be highly...

28 October 2020 2,869 1 View

Single Stage deep learning based instance segmentation?

Hi, Is there any single-stage deep learning based instance segmentation method that outputs single channel mask where each pixel indicates instance labels (assume we are considering one class:...

21 September 2020 9,211 4 View

Desired membrane protein band isn't aligned with marker in SDS-PAGE gel. What could be the possible reason?

I am having problems regarding to protein separation via SDS-PAGE. As a newbie in this field, I have tried to optimize the conditions for protein induction. My protein size is in 20-25KDa range...

03 September 2020 8,243 11 View

How to measures validity of KPIs for academic performance?

I have set of questions of key performance indicators (KPIs) for academic performance of different colleges and program of my university. All the responses on each item are in number (File...

04 February 2020 6,393 3 View

Can anyone help me. Need assistance in PCR I couldn't amplify HVR1 of AMA1 gene in P. falciparum i am following conditions given in Article but still?

I have to amplify HVR1 of AMA1 in Plasmodium falciparum isolates...Annealing temperature given in articles is 55 o C and i am also following all the conditions but still i didn't get positive...

19 February 2019 8,075 3 View

We are using UV Spectroscopy to identify DNA Damage cultured in Broth containing formalin. What methods can be used for identifiying the damage?

We have cultured E. coli in Broth containing formalin. We also measured the growth concentration by Uv Spectroscopy. Our target is to identify DNA damage and DNA mutation location.

24 February 2018 7,546 1 View

Why is it important to distribute questionnaires in double the amount than calculated sample size?

Why it is important to distribute questionnaires double in amount than calculated sample size? e.g. If the calculated sample size is 300, then why it it is necessary to double up the sample e.g....

14 June 2015 7,683 10 View

How to get experience in R?

I am frequent user of SPSS and Stata, I know much about SAS. On the other hand, I know the significance of R too. I learnt and run a few commands based on topics, such as kappa analysis,...

29 November 2012 8,008 24 View

Not getting PCR results

I need your valuable suggestion in my Research I have to amplify HVR-1 of malarial patients infected with Plasmodium falciparum For which i have to perform two PCR Primary and Nested PCR The...

01 January 1970 4,414 33 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View