I am trying to build an amide bond between COOH functionalized aptamer and small molecule. On that purpose I need EDC/NHS solution for establishing amide bond, using MES and PBS buffers, respectively. I did HPLC without exchange of buffers. As a result, i got so many high concentration HPLC peaks at different time durations. One peak that i think usually is my product peak doesn't give me exact LCMS mass. What are other methods for successful coupling of COOH-NH2. Also, what methods can be used for the confirmation of amide bond?

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