How many reaction can I perform by using TAKARA QuickCut Restriction Enzymes Box 1600D?

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If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What is the main factor involve in higher binding score performing molecular docking?

A molecule shows the -11.6 kcal/mol and second one -8.0 kcal/mol so why molecule (A) revealed higher binding score. Please explain major factor involve in it.

21 July 2024 954 5 View

What are the most effective remote sensing techniques and algorithms for detecting LULC changes in rapidly urban area?

What are the most effective remote sensing techniques and algorithms for detecting and analyzing land use and land cover (LULC) changes in rapidly urbanizing areas, and how do their accuracies and...

11 July 2024 6,695 1 View

Students are found bad at listening as they do pretty well in speaking tests as compared with listening tests? How to overcome this problem?

My attempt to promote listening was "Using Short-stories". It works fairly but there could be better options. Role of Short Stories in Developing English Listening Skill

09 July 2024 9,078 4 View

Using Homophones & Homographs is a challenging task for students. Can you please add your ideas and experiences to help them?

I have tried to resolve this issue a little bit, but eager to get your insights and thoughts to make my efforts better. Using Homophones and Homographs in ESL Classrooms

04 July 2024 6,742 3 View

Would you please share your thoughts and insights to bring reforms in 'Distance Education System'?

The contribution of "Distance Education" is immense, but still needs a lot of reforms. Universities generally adopt one another ideas and ignore the ideas of world wide scholars, distance...

04 July 2024 2,545 6 View

Language teachers can develop speaking skill through listening practices. Can you please suggest some more ways to promote the same (Speaking Skill)?

"Today several adjectival phrases have been used to describe English like ‘International Language’, ‘Lingua-Franca’, ‘Language for Globally Connecting’, ‘Library Language’, ‘Official Language’,...

01 July 2024 8,406 5 View

How to develop English listening Skill among Hindi/Urdu Speaking students?

In educational institutions where English is taught as a second language, only 25% of listening happens in English but rather 75% of listening takes place in other language like Hindi/Urdu/Punjabi...

28 June 2024 6,353 4 View

Cut PDMS sheet with Graphtec cutter plotter CE6000-40?

Dear All, I want to cut PDMS sheet with thickness of 0.8 mm to rectangular shape with Graphtec cutter plotter CE6000-40. What condition do you offer? Do you offer any kind of special speed or force?

25 June 2024 8,111 1 View

How do we identify plant compounds by using thin layer chromatographic techniques?

How do we compare the Rf values of the spots to known standards or literature values to help identify the compounds. How TLC does allows the identification and analysis of the mixture of...

07 June 2024 1,967 5 View

Gromacs first step of minimization ?

I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...

07 August 2024 2,598 1 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How we can use lattice-based cryptography for construction of S boxes?

Please suggest basic literature on lattice-based cryptography. A kind response from the cryptographic community will be highly appreciated.

24 July 2024 6,291 4 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

Assuming that the central philosophy of the Schrodnger equation is the cat in a box paradox, can this phylosipy be adjusted?

We assume that the cat in a box paradox may allow an additional approach to include multiple cats in the same box. We can now see two seemingly different views, quantum and classical. The...

13 July 2024 8,233 4 View

Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,255 3 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View

Hello friends In simulating the drug with the liposome, which side of the liposome membrane is the drug placed on? The top leaf or the other?

Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...

29 June 2024 9,688 1 View