Is there a program/code for distinguishing between plants in thermal camera imaging?

My research includes monitoring canopy temperature over time with a thermal FLIR camera. I am looking for a code/program that will help me analyze the videos easily and automatically. For my...

24 February 2020 4,722 3 View

How to group wells data from growthcurver package?

Hi, I've been using growthcurver package for growth curves experiment. I have the output data per well- but now I want to group wells together according to my meta-data (for instance three...

21 June 2019 6,635 1 View

How to measure rRNA amount?

Hi all, I try to figure out weather there is a change in my rRNA following treatment. when I treat the cells (mouse cells) and isolated RNA, I see a major difference in RNA concentration, without...

06 September 2018 724 7 View

Why I get no amplification using degenerative PCR?

Hello, I'm trying to amplify a gene in both eDNA and bacterial DNA (a strain of Bacillus, that should be the positive control) using degenerative primers, their sequence was published in a...

10 December 2017 503 3 View

What are the best bone marrow derived osteocytes and chondrocytes flow cytometry markers?

I am trying to identify by flow cytometry osteogenic and chondrogenic lineages differentiated from MSCs in human BM. What would be the best markers (intracellular or extracellular) for this...

27 September 2017 7,371 3 View

Anyone knows protocol for viva spin tubes?

24 July 2016 5,940 3 View

Could anyone suggest a dye used in flow cytometry that can stain specifically to RNA but not DNA?

I want to show RNA delivery between cells using stained RNA.

09 September 2015 5,115 3 View

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02 March 2021 3,060 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

No title

28 February 2021 9,936 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View