I have bought a bacteria counting kit for flow cytometry from invitrogen. Every time I have used it on two different species of bacteria, but every time I get two staining populations, one is high staining and clear from the noise, and one intermediate population, which can't be distinguished from the noise. I have tried staining for longer time, but I get same result and makes it impossible to actually count how many bacteria I have in the sample.
Has anyone had similar problems, suggestions are welcome?
Evan Chiswick
I have used that kit and have experience something similar. I noticed that when I dilute the bacteria further, this results in much better separation. I usually run a 3-log dilution range. Acquire cells at the slowest rate (i.e. lo).
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