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When I isolate Xen41 (Pseudomonas Aeruginosa PAO1 with Luciferase system) from LB/tetracycline plates, place into saline, and read the O.D., the values are not stable and actually decrease. (e.g....
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Luminex, and several publications, report that carboxylated polystyrene beads should be activated with EDC:Sulfo-NHS in pH 6.2 phosphate buffer, and then coupled with antibody in pH 5.0 MES...
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I plan to sort peripheral blood neutrophils from septic mice to determine differential gene expression between groups. The sorting will be done on a BD ARIA II flow sorter. How are cells typically...
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In setting the gates for positive events via Fluorescence Minus One (FMO) controls, do you include an isotype matched antibody in the FMO cocktail, or leave that channel empty altogether? I.e.:...
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