I have looked for a commercial or private source of NB4 cells and have not had luck at either the ATCC or DSMZ. Does anyone know of either a commercial source or investigator that produces NB4 cells? The literature suggests that many labs are working with them to study granulocyte differentiation but without mentioning an obvious source.
https://www.ncbi.nlm.nih.gov/pubmed/1995093
http://www.creative-bioarray.com/NB-4-CSC-C0328-item-1139.htm?gclid=CLr5rZPFn9YCFcy37QodoJsC2Q
NB4 data sheet
Details: ACC-207 27/10/2016, 22:30
Cell line:
DSMZ no.: Species: Cell type: Origin:
Reference(s): Biosafety level:
Permissions and restrictions:
Morphology:
Medium: Subculture:
Incubation:
Doubling time: Harvest:
Storage:
Mycoplasma: Immunology: Fingerprint:
NB-4 ACC 207 human (Homo sapiens) acute promyelocytic leukemia
established from the bone marrow of a 23-year-old woman with acute promyelocytic leukemia (APL = AML FAB M3) in second relapse in 1989; patented cell line; cells carry the t(15;17) PML-RARA fusion gene
14480 , 14496 , 14705
1
A
DSMZ Cell Culture Data:
single cells in suspension, round and polymorphic cells, very few are giant cells; image ; image ; image
90% RPMI 1640 + 10% h.i. FBS
maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio at 1:2 to 1:3 every 2-3
days; seed out at ca. 0.5-1 x 106 cells/ml; viability may be low after thawing, but cells will recover soon when cultured initially with 20% FBS; cells might also grow better in 24-well-plates than in culture flasks
at 37 °C with 5% CO2 ca. 35-45 hours
cell harvest of 1.5-2.0 x 106 cells/ml
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
CD3 -, CD4 +, CD11b -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 - , CD38 +, HLA-DR -; image
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: Cytogenetics:
Molec. Genetics: Viruses:
Supplied as:
confirmed as human with IEF of AST, MDH
human hypertriploid karyotype with 3% polyploidy - 78(71-81)XX, -X, +2, +6, +7, +7, +11, +12, +13, +14, +17, -19, +20, +4mar, der(8)t(8;?) (q24;?), der(11)t(11;?)(?->::11p15->11q22.1::11q13->22.1:), der(12)t(12;?) (p11;?), 14p+, t(15;17)(q22;q11-12.1), der(19)t(10;19)(q21.1;p13.3)x2 - identity confirmed
expression of fusion gene PML-RARA (L-form) confirmed by RT-PCR
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 - , HIV -, HTLV-I/II -, MLV -, SMRV
Regards,
JP
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